Water quality fecal coliform standard quality control sample (multi-tube fermentation method)|BNCC360422 |BNCC

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Water quality fecal coliform standard quality control sample (multi-tube fermentation method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Samples require a total of 60mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biological safety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of it is transferred to the above sterile bottle, which is a standard quality control sample with a total concentration of 60mL and a concentration of 300 ~ 8000MPN/L.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	1
application	Water quality fecal coliform determination quality control and personnel assessment (multi-tube fermentation method). Applicable Standard: HJ 347.2-2018 Water Quality Determination of Fecal Coliforms Multi-tube Fermentation Method
Sharing mode	Public welfare sharing

water quality fecal coliform standard quality control sample certificate

1. Product information:

Sample name: water quality fecal coliform standard quality control sample (multi-tube fermentation method)

Sample number: BNCC 360422

sample batch: 211203

2. Product features:

description	appearance is white powder (freeze-dried powder), containing protective agent, 7mL penile bottle packaging
traceability	ATCC 25922 Escherichia coli, ATCC Enterobacter aerogenes 13048
concentration value	300~8000MPN/L
reference value	1700MPN/L
uniformity and stability	Random sampling, good uniformity of results; long-term stability test, stability qualified applicable method
applicable method	"HJ 347.2-2018 water quality determination of fecal coliform multi-tube fermentation method"
* Note: If you have any questions about quality control samples, please contact BNCC for help

3. Instructions

Dissolve the material by60mL of sterile water or sterile saline. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3-4ml of sterile water is immediately added, dissolved and transferred into a sterile bottle; rinse the inner wall of the penicillin bottle with the remaining sterile water, and transfer all the solution to the former sterile bottle. That is reference quality control material，totaling 60ml, with a concentration of 300~8000MPN/L。

4. Precautions

1. Upon receipt, please keep it properly. if it is damaged, please contact us within 24 hours.

2. Please handle in a biosafety cabinet under sterile conditions. The material shall be used up once opened.

3. the product contains risk-3 pathogenic organisms，please do not directly touch the samples with your hands, please discard the residual samples after high temperature and high pressure sterilization.

5.Preservation and transportation

long-term storage: -20 ℃, valid for 1 year; ship on ice bag; the shipping time should not exceed 10 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc .org .cn tel: 400-6699-833

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