Water quality total coliform negative quality control sample (paper rapid method)|BNCC360413 |BNCC

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Water quality total coliform negative quality control sample (paper rapid method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Samples require a total of 6 mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30 min, then opened in the biosafety cabinet, 6 mL of sterile water is immediately added, and the vials are shaken after dissolution, and the uniform shaking is the negative quality control sample, with a total concentration of 6 mL and a concentration of 300~3000 CFU/mL.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	2
application	Water quality total coliform and fecal coliform group paper rapid detection quality control and personnel assessment
Sharing mode	Public welfare sharing

water quality total coliform negative quality control sample certificate

1. Product information

Sample name: water quality total coliform negative quality control sample (paper rapid method)

Sample number: BNCC 360413

sample batch: 211203

2.Product features

description	appearance is white powder (freeze-dried powder), containing protective agent, 7mL penile bottle packaging
traceability	ATCC 6538 Staphylococcus aureus
concentration value	300~3000 CFU/mL
reference value	962 CFU/mL
uniformity and stability	Random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	"HJ 755-2015 Rapid Paper Method for Determination of Total Coliforms and Fecal Coliforms in Water Quality"
* Note: If you have any questions about quality control samples, please contact BNCC for help

3. Instructions

Samples require a total of 6 mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 30 minutes, then open it in a biological safety cabinet, immediately add 6 mL of sterile water, shake the vial after dissolving, and shake it evenly to obtain a negative quality control sample with a concentration of 300~3000 CFU /mL.

4. Precautions

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt.

2. Please operate in a sterile environment and in a biological safety cabinet. After opening, you must use it all at once.

3. This product contains three types of pathogenic microorganisms. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them.

5. Preservation and transportation

Long-term storage: -20℃, valid for 1 year; transportation conditions: ice pack transportation, the time should not exceed 10 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

Website: www.bncc .org .cn Tel: 400-6699-833

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