Water Quality Total Coliform Standard Quality Control Sample (Paper Rapid Method)|BNCC360407 |BNCC

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Water Quality Total Coliform Standard Quality Control Sample (Paper Rapid Method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	The sample requires a total of 100 mL of sterile water or sterile normal saline to dissolve. Before use, the quality control sample is placed at room temperature for 30 min, then opened in the biological safety cabinet, 3-4 mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is the standard quality control sample with a total concentration of 100 mL and a concentration of 300-15000 MPN/L.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	2
application	Water quality total coliform and fecal coliform group paper rapid detection quality control and personnel assessment
Sharing mode	Public welfare sharing

water quality total coliform standard quality control sample certificate

1. Product information

Sample name: water quality total coliform standard quality control sample (paper rapid method)

Sample number: BNCC 360407

sample batch: 211203

2. Product features

description	appearance is white powder (freeze-dried powder), containing protective agent, 7mL penile bottle packaging
traceability	ATCC 25922 Escherichia coli, ATCC 6538 Staphylococcus aureus
concentration value	300~15000 MPN/L
reference value	700 MPN/L
uniformity and stability	Random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	"HJ 755-2015 Rapid Paper Method for Determination of Total Coliforms and Fecal Coliforms in Water Quality"
* Note: If you have any questions about quality control samples, please contact BNCC for help

3.Instructions

Dissolve the material by 100mL of sterile water or sterile saline. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3-4ml of sterile water is immediately added, dissolved and transferred into a sterile bottle; rinse the inner wall of the penicillin bottle with the remaining sterile water, and transfer all the solution to the former sterile bottle. that is reference quality control material，the concentration is 300~15000 MPN/L.

4.Precautions

1. Upon receipt, please keep it properly. if it is damaged, please contact us within 24 hours.

2. Please handle in a biosafety cabinet under sterile conditions. The material shall be used up once opened.

3. the product contains risk-3 pathogenic organisms，please do not directly touch the samples with your hands, please discard the residual samples after high temperature and high pressure sterilization.

5. Preservation and transportation

long-term storage: -20 ℃, valid for 1 year; ship on ice bag; the shipping time should not exceed 7 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc .org .cn tel: 400-6699-833

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