Water Quality Fecal Coliform Standard Quality Control Sample (Paper Rapid Method)|BNCC360406 |BNCC

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Water Quality Fecal Coliform Standard Quality Control Sample (Paper Rapid Method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	The sample requires a total of 100 mL of sterile water or sterile normal saline to dissolve. Before use, the quality control sample is placed at room temperature for 30 min, then opened in the biological safety cabinet, 3-4 mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is the standard quality control sample with a total concentration of 100 mL and a concentration of 300-15000 MPN/L.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	1
application	Water quality total coliform and fecal coliform group paper rapid detection quality control and personnel assessment
Sharing mode	Public welfare sharing

water quality fecal coliform standard quality control sample certificate

1. Product information

Sample name: water quality fecal coliform standard quality control sample (paper rapid method)

Sample number: BNCC 360406

sample batch: 211203

2. Product features

description	appearance is white powder (freeze-dried powder), containing protective agent, 7mL penile bottle packaging
traceability	ATCC 25922 Escherichia coli, ATCC Enterobacter aerogenes 13048
concentration value	300~15000 MPN/L
reference value	790 MPN/L
uniformity and stability	Random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	"HJ 755-2015 Rapid Paper Method for Determination of Total Coliforms and Fecal Coliforms in Water Quality"
* Note: If you have any questions about quality control samples, please contact BNCC for help

3. Instructions

Samples require a total of 100 mL of sterile water or sterile saline to dissolve. Before use, put the quality control sample at room temperature for 30 minutes, then open it in the biological safety cabinet, immediately add 3~4 mL of sterile water, dissolve it, suck it out and put it into a sterile bottle, and repeatedly wash the inner wall of the vial with the remaining sterile water. , all transferred to the above sterile bottle, that is, the standard quality control sample, the concentration is 300~15000 MPN/L.

4. Precautions

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt.

2. Please operate in a sterile environment and in a biological safety cabinet. After opening, you must use it all at once.

3. This product contains pathogenic microorganisms. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them.

5. Preservation and transportation

Long-term storage: -20℃, valid for 1 year; transportation conditions: ice pack transportation, the time should not exceed 10 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

Website: www.bncc .org .cn Tel: 400-6699-833

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