Water quality enzyme substrate method quality control sample|BNCC360391 |BNCC

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Water quality enzyme substrate method quality control sample

Culture medium	MMO-MUG medium: composition (g/L): ammonium sulfate 5.0; Manganese sulfate 0.0005; Zinc sulfate 0.0005; Magnesium sulfate 0.1; Sodium chloride 10.0; Calcium chloride 0.05; Sodium sulfite 0.04; Amphotericin B 0.001; ONPG 0.5;MUG 0.075;Solanium extract 0.5;HEPES 6.9;HEPES sodium salt; Take 100ml of water sample from a 100ml sterile dilution bottle and add 2.7±0.5g of this product, shake evenly to completely dissolve it, and put it into an incubator at 36±1 ℃ for 24 hours.
Subculture procedure	Before use, the quality control sample is placed at room temperature for 30min, and the sample needs to be dissolved in a total of 100mL of sterile water or sterile normal saline. Then open it in the biological safety cabinet, immediately add 3~4mL of sterile water, dissolve it and suck it out into a sterile bottle, repeatedly wash the inner wall of the penile bottle with the remaining sterile water, and transfer it to the above sterile bottle, which is 100mL standard sample stock solution.
Growth conditions	37 ℃, aerobic; 44.5 ℃, aerobic;
Storage conditions	-20 ℃, cold storage away from light; Ice bag transportation
Safety level	2
application	Reference standard: HJ 1001-2018 Enzyme Substrate Method for Determination of Total Coliforms, Fecal Coliforms and Escherichia coli in Water Quality; It is used for the quality control of enzyme substrate method for detection of total coliforms, fecal coliforms and Escherichia coli. The number of strains required for production is BNCC269342, BNCC286903 and BNCC238481.
Sharing mode	Public welfare sharing

water quality enzyme substrate method quality control sample certificate

1. Product information

Sample name: water quality enzyme substrate method quality control sample

Sample number: BNCC 360391

sample batch: 211203

2. Product features

description	appearance is white powder (freeze-dried powder), containing protective agent, 7mL penicillin bottle packaging
traceability	ATCC 25922 Escherichia coli, ATCC Enterobacter aerogenes 13048, ATCC 6538 Staphylococcus aureus
concentration value	300~8000 MPN/L
reference value	total coliform group 7270 MPN/L
	fecal coliform 3811 MPN/L
	Enterocephalus 1354 MPN/L
uniformity and stability	Random sampling, good uniformity of results; Long-term stability test, stability qualified
specific use	for quality control of enzyme substrate detection of total coliforms, fecal coliforms and Escherichia coli in water quality
applicable standards	《HJ 1001-2018 Enzyme Substrate Method for Determination of Total Coliform, Fecal Coliform and Escherichia coli in Water Quality》
* Note: If you have any questions about quality control samples, please contact BNCC for help

3. Instructions

Dissolve the material by 100mL of sterile water or sterile saline. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3-4ml of sterile water is immediately added, dissolved and transferred into a sterile bottle; rinse the inner wall of the penicillin bottle with the remaining sterile water, and transfer all the solution to the former sterile bottle. That is the standard sample stock solution.

4. Precautions

1. Upon receipt, please keep it properly. if it is damaged, please contact us within 24 hours.

2. Please handle in a biosafety cabinet under sterile conditions. The material shall be used up once opened.

3. this product contains pathogenic microorganisms, please do not directly touch the samples with your hands, please discard the residual samples after high temperature and high pressure sterilization.

5. Preservation and transportation

long-term storage: -20 ℃, valid for 1 year; ship on ice bag; the shipping time should not exceed 7 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc .org .cn tel: 400-6699-833

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