Standard quality control sample of fecal coliform (filter membrane method)|BNCC359578 |BNCC

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Standard quality control sample of fecal coliform (filter membrane method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Samples require a total of 500mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biological safety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is the standard quality control sample, equivalent to 500mL of water sample to be tested. It is suggested that the filtration volume is 25~65mL, and the number of fecal coliform colonies is 20~60, and the total number is less than 200.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	1
application	Suitable for water quality fecal coliform determination quality control and personnel assessment (filter membrane method). Reference Standard "HJ 347.1-2018 Water Quality Determination of Fecal Coliform Bacteria Filter Method"
Sharing mode	Public welfare sharing

Fecal coliform standard quality control sample certificate

1. Product information:

Sample name: fecal coliform standard quality control sample (filter method)

Sample number: 359578

sample batch: 210823

2. Product features:

description	appearance is white powder (freeze-dried powder), containing protective agent, 5mL penicillin bottle packaging
traceability	ATCC 25922 Escherichia coli, ATCC 13048 Enterobacter aerogenes
Concentration value	positive 50 ~ 300CFU/L, negative 50 ~ 300CFU/L
Stability and uniformity	Random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	"HJ 347.1-2018 water quality fecal coliform determination filter method"
* note: if you have any questions about quality control samples, please contact the center (BNCC) for help before use.

3. Instructions

Dissolve the material by 100mL of sterile water or sterile saline. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3-4ml of sterile water is immediately added, dissolved and transferred into a sterile bottle; rinse the inner wall of the penicillin bottle with the remaining sterile water, and transfer all the solution to the former sterile bottle. That is the standard sample stock solution,Equal to 500mL of water sample to be tested. The recommended filtration volume is 25~65mL, then the number of fecal coliform colonies is 20~60, and the total number is less than 200.

4.Notes

1. Upon receipt, please keep it properly. if it is damaged, please contact us within 24 hours.

2. Please handle in a biosafety cabinet under sterile conditions. The material shall be used up once opened.

3. this product contains pathogenic microorganisms, please do not directly touch the samples with your hands, please discard the residual samples after high temperature and high pressure sterilization.

5. Preservation and transportation

long-term storage: -20 ℃, valid for 2 year; ship on ice bag; the shipping time should not exceed 10 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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