Fecal coliform negative control samples (filter method)|BNCC359577 |BNCC

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Fecal coliform negative control samples (filter method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Samples require a total of 500mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is a negative quality control sample with a total concentration of 500mL and a concentration of 40 ~ 600CFU/L.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	1
application	Suitable for water quality fecal coliform determination quality control and personnel assessment (filter membrane method). Reference Standard "HJ 347.1-2018 Water Quality Determination of Fecal Coliform Bacteria Filter Method"
Sharing mode	Public welfare sharing

Fecal Coliform Negative Quality Control Sample Certificate

1. Product information:

Sample name: Fecal coliform negative control sample (filter method)

Sample number: 359577

sample batch: 210823

2. Product features:

description	appearance is white powder (freeze-dried powder), containing protective agent, 5mL penicillin bottle packaging
traceability	ATCC 13048 Enterobacter aerogenes
concentration value	40~600CFU/L
uniformity and stability	Random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	《HJ 347.1-2018 water quality fecal coliform determination filter method》
* note: if you have any questions about quality control samples, please contact the center (BNCC) for help before use.

3.Instructions

Dissolve the material by 500mL of sterile water or sterile saline. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3-4ml of sterile water is immediately added, dissolved and transferred into a sterile bottle; rinse the inner wall of the penicillin bottle with the remaining sterile water, and transfer all the solution to the former sterile bottle. It is the negative quality control sample, with a total of 500mL and a concentration of 40~600CFU/L

4. Precautions

1. after receiving the goods, please keep it properly. if it is damaged, please contact the center within 24 hours after receiving it.

2. Please operate in a sterile environment and a biosafety cabinet when using. After opening, you must use it all at once.

3. this product contains three types of pathogenic microorganisms, please do not directly touch the samples in the bottle with your hands, please discard the residual samples after high temperature and high pressure sterilization.

5. Preservation and transportation

long-term preservation:-20 ℃, valid for 2 years;

Transportation conditions:shipment on ice bag within 10 days

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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