Fecal coliform positive control samples (membrane method)|BNCC359576 |BNCC

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Fecal coliform positive control samples (membrane method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Samples require a total of 500mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is a positive quality control sample with a total concentration of 500mL and a concentration of 40 ~ 600CFU/L.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	1
application	Suitable for water quality fecal coliform determination quality control and personnel assessment (filter membrane method). Reference Standard "HJ 347.1-2018 Water Quality Determination of Fecal Coliform Bacteria Filter Method"
Sharing mode	Public welfare sharing

Fecal Coliform Positive Quality Control Sample Certificate

1. Product information:

Sample name: Fecal coliform positive control sample (filter method)

Sample number: 359576

sample batch: 210823

2. Product features:

description	appearance is white powder (freeze-dried powder), containing protective agent, 5mL penicillin bottle packaging
traceability	ATCC 25922 Escherichia coli
concentration value	40~600CFU/L
uniformity and stability	Random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	《HJ 347.1-2018 water quality fecal coliform determination filter method》
* note: if you have any questions about quality control samples, please contact the center (BNCC) for help before use.

3. Instructions

Dissolve the material by 500mL of sterile water or sterile saline. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3-4ml of sterile water is immediately added, dissolved and transferred into a sterile bottle; rinse the inner wall of the penicillin bottle with the remaining sterile water, and transfer all the solution to the former sterile bottle. It is the positive quality control sample, with a total of 500mL and a concentration of 40~600CFU/L

4.Notes

1. Upon receipt, please keep it properly. if it is damaged, please contact us within 24 hours.

2. Please handle in a biosafety cabinet under sterile conditions. The material shall be used up once opened.

3. this product contains pathogenic microorganisms, please do not directly touch the samples with your hands, please discard the residual samples after high temperature and high pressure sterilization.

5. Preservation and transportation

long-term storage: -20 ℃, valid for 2 year; ship on ice bag; the shipping time should not exceed 10 days.

Henan Engineering Research Center of Industrial Microbial Strain

website: www.bncc .org .cn tel: 400-6699-833

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