spore suspension|BNCC358969 |BNCC

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spore suspension

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then pack and freeze-dry;
Growth conditions	30 ℃;18-24h; Aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, round, yellow on the front, light in color, convex in the middle, bright and opaque surface, neat edges, smooth surface, moist texture, G + (blue-purple), bacilli
application	Resistance Testing Dry heat sterilization control Efficacy testing Ethylene oxide sterilization control Media testing Steam sterilization control Sterility assurance Sterilization control Pharmaceutical and Personal Care Resistance test dry heat sterilization control efficacy test ethylene oxide sterilization control medium inspection BR> sterility assurance sterilization control> drugs and personal care
Sharing mode	Public welfare sharing

Bacillus subtilis black variety spore suspension certificate

1. Product information:

Sample name: Bacillus subtilis black variety spore suspension

Sample number: 358969

Sample atch: 220325

2. Product features:

Description	the appearance is 5mL penile bottle packaging, containing spore suspension, turbid liquid, placed with precipitation.
Traceability	ATCC 9372 Bacillus subtilis black variety
Concentration value	10⁸ CFU/mL
Applicable method	verification of sterilization effect in accordance with dry heat sterilization method "2020 edition disinfection technical specification" and GB/T 33419 Evaluation, etc.
* Note:If you have any questions about the product, please contact BNCC for help before use.

3. Instructions

This spore suspension is pure spore suspension, which is obtained by cell separation and collection of spores. it can be used directly and the corresponding volume can be added according to the test requirements.

4. Notes:

1. Please keep it properly after receiving the goods, if any damaged is found , please contact the customer service within 24 hours.

2. Please operate in a sterile environment and a biosafety cabinet when using. You must use it all at once after opening.

3. This microbial live bacteria product has certain biosafety risks. please do not directly touch the samples in the bottle with your hands. The residual samples after using should be discarded after high temperature and high pressure sterilization.

5. Preservation and transportation long-term preservation:

2-8 ℃, valid for 1 year; Transportation conditions: ice bag transportation, the time should not exceed 10 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc .org .cn ; Tel: 400-6699-833

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