BeNa Culture Collection

Quantitative strain of Bacillus subtilis-BNCC
Quantitative strain of Bacillus subtilis-BNCC
  • BNCC
  • Quantitative strain of Bacillus subtilis-BNCC
  • Quantitative strain of Bacillus subtilis-BNCC

Quantitative strain of Bacillus subtilis

  • Price: $286
  • number:BNCC361420
  • Packing:penicillin bottle;(5.4±0.5)× 10 6CFU/bottle
  • Form:
    The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, bright surface, smooth surface, moist texture, easy to stir up, G + (blue-purple), bacilli, purity: pure
Essential Information Certificate Related Products
Quantitative strain of Bacillus subtilis
Culture medium Tryptone soybean medium (English name: TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions 30 ℃;18-24h; Aerobic;
Storage conditions -20 ℃
morphology The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, bright surface, smooth surface, moist texture, easy to stir up, G + (blue-purple), bacilli, purity: pure
Sharing mode Public welfare sharing

Bacillus subtilis subsp.

Strain number: BNCC 361420 /ATCC6633

Product format: quantitative bacteria tablets

Number of viable bacteria: (5.4±0.5)×106CFU/bottle

Product batch: 220428

Valid until: 2023.04.27

Date of inspection: 2022.04.27

Sampling ratio:≥2%

Report number: RS22293727-1

Biosafety  level:  1, handle in ultra-clear table or safety cabinet

items description results
stain microscopy Gram-negative, positive, cell morphology observed under microscope G + cocci
colony morphology Observe the morphology of single colonies off-white, flat, rough, rounded or irregular edges
purity Non-selective culture medium, colony observation and microscopy

No contaminated bacterial

Reference value of live bacteria Non-selective media plates, viable count 5.4×106CFU/bottle
Phenotypic and Genotypic Analysis Phenotypic testing, gene sequencing qualified
test conclusion After the BNCC quality control inspection system, the product is qualified

Product list: (2.0±0.5)×10^7CFU quantitative strains 1 bottle, 1mL lysate 1 bottle, 1 strain certificate;

Validity period: unopened, below -20 ℃, 24 months.

Usage:

1. Open the strain and lysate according to the arrow indication of the vial cap;

2. Pour the bacterial flakes into the dissolving solution, cover with a rubber stopper, and shake to dissolve;

3. After fully dissolved, it is (5.4±0.5)×106CFU/mL, and it can be drawn in proportion according to the test requirements;

4. Please use up immediately after opening and do not store;

Notes:

① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.

②Used germ-carrying consumables should be discarded after autoclaving.

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