Quantitative strain of Enterobacter aerogenes|BNCC356329 |BNCC

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Quantitative strain of Enterobacter aerogenes

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	1. According to the arrow sign of penicillin bottle cap, open the strain and dissolved liquid; 2. transfer the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After full dissolution, it is 100 ~ 500CFU/ml, which is absorbed in proportion according to the requirements; 4. Please use it up immediately after opening and do not store it;
Growth conditions	37 ℃;18-24h; Aerobic;
Storage conditions	-20 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, yellow on the front, light color, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G-(red), bacilli
Separation substrate	Sputum
application	Type strain. Water quality testing, quality control strains.
Sharing mode	Public welfare sharing

Enterobacter aerogenes

Strain number:BNCC 358452 / CMCC(F)98001

Name: Enterobacter aerogenes

Product form: quantitative bacteria tablets Number of viable bacteria:100~500CFU/bottle

Product batch:22012501 Valid until:2023.01.24

Inspection date: 2022.01.26 Sampling ratio: ≥2%

Report number:RS22356329-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

Item	description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli
colony morphology	observation of single colony morphology	Light yellow, moist, raised, with neat edges
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	240CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	qualified

Product list: 100 ~ 500CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

Validity period: unopened, below -20 ℃, 12 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After fully dissolved, it is 100 ~ 500CFU/mL, which can be absorbed in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

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