Quantitative strain of Bacillus cryoides|BNCC356043 |BNCC

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Quantitative strain of Bacillus cryoides

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	30 ℃;18-24h; Aerobic;
Storage conditions	-80 ℃
morphology	Purity: pure
application	Biological indicators of hot air disinfection control; L-desoxynojirimycin generation
Sharing mode	Public welfare sharing

Bacillus atrophaeus

Strain number: 356043

Name: Bacillus atrophaeus

Product format: quantitative bacteria tablets Number of viable bacteria: (3.0± 1.0)×10^6CFU/bottle

Product batch: 220418 Valid until: 2024.04.17

Inspection date: 2022.04.18 Sampling ratio: ≥2%

Report number: RS22356043-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

project	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli
colony morphology	observation of single colony morphology	Yellow, raised, bright, moist
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	3.0×10^6CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

Product list:1 bottle of (3.0± 1.0)×10^6CFU quantitative strain, 1 bottle of 1mL dissolved solution and 1 copy of strain certificate;

Shelf life: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After full dissolution, it is (3.0± 1.0)×10^6CFU/mL, can be absorbed in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

(2) Used bacteria-carrying consumables, please autoclave and discard them.

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