Quantitative strain of Streptococcus salivarius|BNCC354947 |BNCC

Essential Information Certificate Related Products

Quantitative strain of Streptococcus salivarius

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	1. According to the arrow sign of penicillin bottle cap, open the strain and dissolved liquid; 2. transfer the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After full dissolution, it is (2.5±1.0)× 10 6cfu/ml, which is absorbed in proportion according to the requirements; 4. Please use it up immediately after opening and do not store it;
Growth conditions	37 ℃; Aerobic;
Growth characteristics	Gram-positive bacteria, spherical, chain-like arrangement, fermenting glucose to produce lactic acid.
Storage conditions	-80 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, smooth and bright surface, moist texture, easy to pick up, G + (blue-purple), cocci, purity: pure
Separation substrate	blood - human
application	Type strain, fermentation of glucose to produce lactic acid
Sharing mode	Public welfare sharing

Streptococcus salivarius

Strain number: BNCC 354947

Name: Streptococcus salivarius

Product format: quantitative bacteria tablets Number of viable bacteria: (2.5±1.0)×10^6CFU/bottle

Product batch: 21122001 Valid until: 2022.12.19

Inspection date: 2021.12.21 Sampling ratio: ≥2%

Report number:RS21354947-1 Biosafety level: 1,handle in ultra-clear table or safety cabinet

Item	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G + cocci, spherical, chain-like arrangement
colony morphology	observation of single colony morphology	pale yellow, raised, moist, bright
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	2.0×10^6CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

Product list: One bottle of (2.5±1.0)×10^6CFU quantitative strain, one bottle of 1mL dissolved solution and one copy of strain certificate;

Validity period: unopened, below -20 ℃, 12 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After full dissolution, it is (2.5±1.0)×10^6CFU/bottle, according to the test requirements according to the proportion of suction can be;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

Download certificate