Salmonas salinaria|BNCC361171 |BNCC

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Salmonas salinaria

Culture medium	2216E seawater agar (English name: 2216E): peptone 5.0g, yeast extract 1.0g, ferric citrate 0.1g, potassium bromide 0.08g,NaCl 19.45g,MgCl2 8.8g,Na2SO4 3.24g,CaCl2 1.8g,KCl 0.55g,NaHCO3 0.16g,NH4NO3 0.0016g,Na2HPO4 0.008g, strontium chloride 0.034g, boric acid 0.022g, sodium silicate 0.004g, pH 7.4-7.8. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;24-48h; Aerobic
Storage conditions	2-8 ℃
morphology	The colony diameter is 0.5-1mm, round, opaque, gray-white on the front, convex in the middle, bright surface, moist texture, easy to stir up, G-(red), bacilli
Separation substrate	Mud water
Sharing mode	Public welfare sharing

Halomonas salifodinae

No. 361171

Product format: freeze dried,200ul

Storage conditions: 2~8 ℃

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 30℃, aerobic, 2216E seawater agar medium, 24-48h. 2216E seawater agar medium: peptone 5.0g, yeast extract 1.0g, ferric citrate 0.1g, potassium bromide 0.08g, NaCl 19.45g, MgCl2 8.8g, Na2SO4 3.24g, CaCl2 1.8g, KCl 0.55g, NaHCO3 0.16g , NH4NO3 0.0016g, Na2HPO4 0.008g, strontium chloride 0.034g, boric acid 0.022g, sodium silicate 0.004g, sodium fluoride 0.0024g, agar 15.0g (not included in liquid medium), distilled water 1000ml, pH 7.4-7.8. Sterilize at 121°C for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 48h, plate bacteria layer is obvious; colony is typical on the streaked plate
Colony morphology: (pictured above)	Size: 0.5-1mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white Protuberance: Middle convex surface: bright texture: moist and easy to stir
conclusion: good viability, no abnormal colony morphology, qualified

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