Verticillium albo-atrum|BNCC360661 |BNCC

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Verticillium albo-atrum

Culture medium	Potato Glucose Agar (English Name: PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;3-5 days; Aerobic
Storage conditions	2-8 ℃
morphology	Spread growth, irregular shape, irregular edges, opaque, pale pink, reverse yellow, light color, rough surface, dry texture
application	Verticillium dahliae of cotton (for determination)
Sharing mode	Public welfare sharing

Verticillium albo-atrum

Storage conditions: 2~8 ℃

No. 360661

Product format; freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28°C, aerobic, potato dextrose agar, 3-5 days. Potato dextrose agar: 1.0L potato cooking liquid, 20.0g glucose, 15.0g agar, natural pH. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open it in the the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 3-5days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5 days plate colonies are obvious
colony morphology:	small filamentous fungi, with obvious colonies on potato glucose agar medium, the prophase of the plate mycelium is white, and gradually It turns pale pink, and the back of the medium is pale yellow.
conclusion: good viability, no abnormal colony morphology, qualified

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