Leptothrix sp.|BNCC360234 |BNCC

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Leptothrix sp.

Culture medium	DSM803 medium (English name: DSM803): yeast extract 1.00g, peptone 1.50g, magnesium sulfate x7H2O 0.20g, calcium chloride 0.05g, ferric ammonium citrate 0.50g,MnSO4 x H2O 0.05g,FeCl3 x 6H2O 0.01g, agar 20.00g, ultrapure water 1000.00ml,pH 7.1,121 ℃,15mim sterilization.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	25 ℃;48-72h; Aerobic;
Storage conditions	2-8 ℃
morphology	Round, neat edges, opaque, yellow on the front, lighter color, convex in the middle, smooth and bright surface, moist texture, easy to pick up, G-(red), bacilli, purity: pure
Separation substrate	soil ginseng field
Sharing mode	Public welfare sharing

Leptothrix sp.

Storage conditions: 2~8 ℃

No. 360234

Product format： freeze dried, 200ul

Validity: 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25 ℃, aerobic, DSM803 medium, 48-72h. DSM803 medium: yeast extract 1.00g, peptone 1.50g, magnesium sulfate x7H2O 0.20g, calcium chloride 0.05g, ferric ammonium citrate 0.50g,MnSO4 x H2O 0.05g,FeCl3 x 6H2O 0.01g, agar 20.00g, ultrapure water 1000.00ml,pH 7.1,121 ℃, 15mim sterilization.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 48hours liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology:	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque color: light yellow uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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