Prevotella histicola Downes et al. 2008|BNCC359116 |BNCC

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Prevotella histicola Downes et al. 2008

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;48-72h; Anaerobic;
Storage conditions	2-8 ℃
morphology	Colony diameter is 1-2mm, round, irregular edge, opaque, front white, dark, convex in the middle, smooth and bright surface, moist texture, easy to stir up, no pigment, G-(red), bacilli, purity: pure
Sharing mode	Public welfare sharing

Prevotella histicola

Storage conditions : 2~8 ℃

No. : 359116

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours in time. if it is overdue, it will be deemed as good receipt. Dry powder shall not be retained after one use, and it can be used after being activated for 1~2 generations. please refer to the attached page for the use of the flat panel. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions:37 ℃, anaerobic, Colombian blood plate (ready-to-use type),24-48h.

Recovery steps:

① Prepare 2 of the above plates;

② After disinfecting the surface of the ampoule tube, open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ Suck about 0.5mL of sterile water (placed in anaerobic environment for 24 hours) and drive it into a freeze-drying tube. after fully dissolving the bacterial powder, drive it into 2 plates, 200μL/piece, evenly coated;

④ Place the plate under the above culture conditions for 24-48h.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability， in 48hours strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque color: white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability, no abnormal colony morphology, qualified

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