Clostridium baishi|BNCC359034 |BNCC

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Clostridium baishi

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;48-72h; Anaerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: white bulge: middle bulge surface: rough texture: moist and easy to stir up
Separation substrate	soil
Sharing mode	Public welfare sharing

Clostridium bergii

Storage conditions : 2~8 ℃

No. : 359034

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if it is overdue, it will be deemed as good receipt. Dry powder shall not be retained after one use, and can be used after activation for 1~2 generations. please refer to the attached page for the use of flat plate and bacterial liquid. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions:37 ℃, anaerobic, Colombian blood plate (ready to use),48-72h ;

Recovery steps:

① Prepare the above flat plate 1-2 block (put it in anaerobic environment for 24 hours in advance);

② Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ Suck 0.5mL of sterile water and put it into the freeze-drying tube. after fully dissolving the bacterial powder, put it into the plate 200μL/piece, coated evenly;

④ Place the plate under the above culture conditions, and the strain can be used when it grows out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability， in 48hours strain layer is obvious;colony is typical on the streaked plate
Colony morphology: (pictured above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: White Protuberance: Middle convex surface: rough texture: moist and easy to stir
conclusion: good viability, no abnormal colony morphology, qualified

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