Yarrowia lipolytica|BNCC358913 |BNCC

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Yarrowia lipolytica

Culture medium	YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;24-48h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: white uplift: flat surface: rough texture: dry
Sharing mode	Public welfare sharing

Yersinia lipolysis

Storage conditions : 2~8 ℃

No. : 358913

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if the goods are overdue, it will be deemed as good. Dry powder shall not be retained after one use, and can be used after activation for 1~2 generations. please refer to the attached page for the use of inclined plane and bacterial liquid. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions:28 ℃, aerobic, YM medium, 24-48h. YM medium: yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 6.2 ± 0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

① Prepare a test tube containing 5~10mL of liquid culture medium and 2 plates;

② Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ Suck 0.5mL of liquid culture medium into freeze-dried tube, fully dissolve and return to liquid test tube again, and mix well;

④ Absorb 0.2mL of bacterial suspension and mix it into the plate, coat it evenly, and repeat it twice to obtain two plates.

⑤ The liquid test tube and the plate are all cultured under the above culture conditions, and the bacteria can be used after growing out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability， in 48hours liquid medium become turbid,strain layer is obvious;colony is typical on the streaked plate
Colony morphology: (pictured above)	Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: White Protuberance: Flat Surface: Rough Texture: Dry
conclusion: good viability, no abnormal colony morphology, qualified

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