Lecanicillium aranearum (Petch) Zare & W. Gams|BNCC358106 |BNCC

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Lecanicillium aranearum (Petch) Zare & W. Gams

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies, pale pink, slowly spreading to the edge, and pale yellow on the back of the medium.
Sharing mode	Public welfare sharing

Lecanicillium aranearum

Storage conditions : 2~8 ℃

No. : 358106

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Growth conditions:28 ℃, aerobic, integrated PDA,5-7 days.

Comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±;0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Receiving notices: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours in time. if it is overdue, it will be regarded as good receiving goods. The inclined plane of the test tube can be used directly. in principle, it can be used many times without pollution during the storage period, but its vitality will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Recovery steps:

(1) Prepare 1-2 pieces of PDA plates;

(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) Lay flat the small pieces to the center of the agar plate;

(5)put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the cell recovery are reported as follows:

Item	test results
viability :	good viability， in 7days strain layer is obvious;colony is typical on the streaked plate
colony morphology:	small filamentous fungi, with obvious colonies on comprehensive PDA medium, pale pink, slowly spreading to the edge of the growth, the back of the medium is pale yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified;

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