azomonas megatocytogenes|BNCC357823 |BNCC

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azomonas megatocytogenes

Culture medium	Nitrogen fixing medium (ABM):KH2PO4 0.2g,K2HPO4 0.8g,MgSO4 · 7H2O 0.2g,CaSO4 · 2H2O 0.1g,FeCl3 trace g,Na2MoO4 · 2H2O trace g, yeast extract 0.5g, mannitol 20.0g, agar 15.0g, distilled water 1.0L,pH 7.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28°C;24-48h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: yellow uplift: middle convex surface: bright and smooth texture: moist and easy to stir up
Sharing mode	Public welfare sharing

Azomonas macrocytogenes

Storage conditions : 2~8 ℃

No. : 357823

Product format :freeze dried,200ul

Validity period : 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37℃, aerobic, nitrogen-fixing medium, 24-48h. Nitrogen-fixing medium: KH2PO4 0.2g, K2HPO4 0.8g, MgSO4 7H2O 0.2g, CaSO4 2H2O 0.1g, FeCl3 trace g, Na2MoO4 2H2O trace g, yeast extract 0.5g, mannitol 20.0g, agar 15.0g, distilled water 1.0L, pH 7.2. Sterilize at 121°C for 15min.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 48h liquid medium become turbid, obvious strain layer occurs on the plate; colony is typical on marked plate
colony morphology (above)	size: 1-2mm shape: round edge: neat transparency: opaque color: yellow uplift: middle raised surface: bright and smooth texture: moist and easy to stir
conclusion	good viability, no abnormal colony morphology, qualified

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