Pelomonas saccharophila|BNCC357819 |BNCC

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Pelomonas saccharophila

Culture medium	R2A agar medium (R2A): tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast extract 0.5g, soluble starch 0.5g, dipotassium bisulfate 0.3g, magnesium sulfate 0.1g, sodium pyruvate 0.3 agar 12 peptone 0.25 glucose 0.5g,pH 7.2±0.2. 121 ℃,15min
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate μL/piece, and spread it evenly; (4) Place the plate under the above culture conditions.
Growth conditions	30°C, aerobic, 2-3 days
Storage conditions	2-8 ℃
morphology	Colony yellow, round and neat, bright and smooth surface, gram-negative bacilli
Sharing mode	Public welfare sharing

Pelomonas saccharophila

Storage conditions : 2~8 ℃

No. : 357819

Product format: freeze dried,200ul

Validity period : 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30℃, aerobic, R2A agar medium, 2-3 days, R2A agar medium: tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast extract 0.5g, soluble starch 0.5g, dipotassium hydrogen sulfate 0.3g , magnesium sulfate 0.1g, sodium pyruvate 0.3, agar 12 peptone 0.25 glucose 0.5g, pH7.2±0.2. Sterilize at 121°C for 15min.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 2 days strain layer become obvious, colony is typical
colony morphology (above)	size: 1-2mm shape: round edge: neat transparency: opaque color: yellow uplift: middle raised surface: bright and smooth texture: moist and easy to stir
conclusion	good viability, no abnormal colony morphology, qualified

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