BeNa Culture Collection

Drechslera cactivora-BNCC
Drechslera cactivora-BNCC
  • BNCC
  • Drechslera cactivora-BNCC
  • Drechslera cactivora-BNCC

Drechslera cactivora

  • Price: $ 172
  • number:BNCC357691
  • Form:
    Small filamentous fungi, with obvious colonies, black, dense and vigorous hyphae on the integrated PDA medium, spreading and growing to the edge of the plate, The back of the medium is pale black.
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Drechslera cactivora
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28°C;2-3 days; Aerobic;
morphology Small filamentous fungi, with obvious colonies, black, dense and vigorous hyphae on the integrated PDA medium, spreading and growing to the edge of the plate, The back of the medium is pale black.
Sharing mode Public welfare sharing

Drechslera cactivora

Storage conditions : 2~8 ℃

No. : 357691

Product format : agar slant in 14mm test tube

Validity period :growing culture, in 30 days

Biosafety  level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions : 28°C, aerobic, integrated PDA, 3 days. Comprehensive PDA agar: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4·7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates; 

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; 

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; 

(4) Lay flat the small pieces to the center of the agar plate;

(5) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability good viability, in 3 days plate colony is obvious
colony morphology

small filamentous fungi with obvious colonies on comprehensive PDA medium, black,

The hyphae are dense and vigorous, spreading to the edge of the plate, and the back of the medium is light black

conclusion good viability, no abnormal colony morphology, qualified 

 

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