Beauveria felina (DC.) J.W. Carmich|BNCC356392 |BNCC

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Beauveria felina (DC.) J.W. Carmich

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 comprehensive PDA plates; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into pieces with aseptic inoculation hoe and inoculation shovel (see attached page),0.5 × 0.5cm2 small square pieces; (4) flat the small square block to the center of the plate and on the agar surface; (5) the plate is being cultured under the above culture conditions.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on the integrated PDA medium, gray-white, wrinkled, and light yellow on the back of the medium.
Sharing mode	Public welfare sharing

Beauveria felina

Storage conditions: 2~8 ℃

No. 356392

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4) Lay flat the small pieces to the center of the agar plate;

(5) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 7 days plate colony is obvious
colony morphology: (above)	Small filamentous fungi have obvious colonies, gray-white, wrinkled, and light yellow on the back of the medium on the integrated PDA medium.
Conclusion	good viability, no abnormal colony morphology, qualified

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