Sporosarcina psychrophila|BNCC356073 |BNCC

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Sporosarcina psychrophila

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	20°C;18-24h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Golden Uplift: Flat Surface: Bright and Smooth Texture: Wet and Easy to Stir
Sharing mode	Public welfare sharing

Sporosarcina psychrophila

Storage conditions: 2~8 ℃

No. 356073

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:20 ℃, aerobic, nutritious gravy agar, 18-24h. Nutritional gravy agar: peptone 5.0g, beef extract 3.0g,NaCl 5.0g, agar 15.0g (not included in liquid medium), distilled water 1.0L,pH7.0. [Note] Add 5mg MnSO4· H2O when culturing Bacillus is beneficial to produce spores.

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability， in 18-24 hours liquid medium become turbid,strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	size: 1-2mm shape: round edge: neat transparency: opaque color: golden uplift: flat surface: bright and smooth texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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