Bacteroides nordii|BNCC354332 |BNCC

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Bacteroides nordii

Culture medium	Improved GAM medium (mGAM): peptone 5.0g,䏡 Peptone 5.0g, soybean peptone 3.0g, yeast powder 2.5g, beef powder 2.2g, digestive serum powder 10.0g, beef liver powder 1.2g, glucose 0.5g, potassium dihydrogen phosphate 2.5g, sodium chloride 3.0g, soluble starch 5.0g,L-cysteine 0.3g,L-arginine 1.0g,L-tryptophan 0.2g, sodium thioglycolate 0.3g,PH 7.3±0.1,121 ℃,15min. note: (add 1ml of sterile 0.1% vitamin K1 solution and 2mg of sterile hemin for every 200ml, and mix well). preparation of sterile hemin solution: weigh appropriate amount of hemin, add appropriate amount of distilled water, and sterilize under high pressure at 116 ℃ for 20-30 minutes.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;2-3 days; Anaerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white Protuberance: Middle convex Surface: Bright and Smooth Texture: Wet and Easy to Stir
Separation substrate	Human peritoneal fluid
Sharing mode	Public welfare sharing

Bacteroides nordii

Storage conditions: 2~8 ℃

No. 354332

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37℃, anaerobic, modified GAM agar, 2-3 days, modified GAM agar: peptone 15.0g, tryptone 10.0g, soy peptone 3.0g, yeast extract powder 5.0g, beef powder 2.0g, beef liver extract Powder 1.2g, soluble starch 0.3g, L-cysteine 0.15g, glucose 3.0g, potassium dihydrogen phosphate 2.5g, digestive serum powder 13.5g, sodium thioglycolate 0.15g, sodium chloride 3.0g, agar 13.0 g (not included in liquid medium), distilled water: 1L, pH 7.0±0.2, 25℃

Additives: 0.02% vitamin K1 solution and hemin must be added

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability in 3 days liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability, no abnormal colony morphology, qualified

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