Candida albicans (Robin) Berkhout|BNCC352028 |BNCC

Candida albicans (Robin) Berkhout

Price： $ 286
number：BNCC352028
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On YEPD agar after 2 days at 25°C, colonies are cream-colored, shiny, and smooth. Older colonies show filaments-like structure at the margin and may have ridges or folders. Cells are ovoid (3.0-6.0 x 4.0-8.0 μm), budding, mostly singly and rarely clustered in young culture. Cells will elongate and form chain-like branched pseudohyphae in older culture.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Candida albicans (Robin) Berkhout

Culture medium	YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Growth conditions	25-28°C; Aerobic;
Storage conditions	-20 ℃
morphology	On YEPD agar after 2 days at 25°C, colonies are cream-colored, shiny, and smooth. Older colonies show filaments-like structure at the margin and may have ridges or folders. Cells are ovoid (3.0-6.0 x 4.0-8.0 μm), budding, mostly singly and rarely clustered in young culture. Cells will elongate and form chain-like branched pseudohyphae in older culture.
Separation substrate	Man with bronchomycosis
Sharing mode	Public welfare sharing

Candida albicans

Storage conditions : -20 ℃

Validity period: 24 months

No. : 352028

Product format: freeze dried, 200ul

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Use : pharmacopoeia, GB 4789, control quality control strain

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Medium 25: glucose 40g, peptone (casein trypsin digest, gastric enzyme digest of animal tissue) 10g, agar 15g, distilled water 1.0L,pH 5.6±0.2. Sterilization at 121 ℃ for 15min.

Growth conditions temperature: 25-28 ℃ gas environment: aerobic

Recovery steps:
(1)Prepare a sterile test tube of 5-10ml liquid medium and 2 pieces of 90mm plates;
(2Ssterilizing of the penicillin-bottle, under aseptic conditions remove the aluminium cap and rubber plug, draw 0.5ml of liquid culture medium into the vial, aspirate to dissolve the bacterial tablets.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)Distribute it well in 200ml/plate; the plates are not allowed to seal by wrapping.
(5)If it does not grow in 18-24 hours, culture is continued until 72 hours is reached. And the strains can be used when they grow

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