Soxhlet phenylacetate|BNCC351924 |BNCC

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Soxhlet phenylacetate

Culture medium	R2A agar medium (R2A): tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast extract 0.5g, soluble starch 0.5g, dipotassium bisulfate 0.3g, magnesium sulfate 0.1g, sodium pyruvate 0.3 agar 12 peptone 0.25 glucose 0.5g,pH 7.2±0.2. 121 ℃,15min
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;3-5 days; Aerobic;
Storage conditions	2-8 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, flat in shape, smooth in surface, bright in surface, moist in texture, easy to stir up, G-(red), bacilli, purity: pure
application	Type strain
Sharing mode	Public welfare sharing

Description

1. Name: Thauera phenylacetica

2. BNCC No.: 351924

3. Biosafety level: 4

2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, R2A agar: tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast powder 0.5g, soluble starch 0.5g, dipotassium bisulfate 0.3g, magnesium sulfate 0.1g, sodium pyruvate 0.3 agar 12 peptone 0.25 glucose 0.5g,pH7.2±0.2. 121 ℃,15min.

2. Atmosphere: aerobic

3, Temperature: 30 ℃,1-5d

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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