Penicillium citrinum|BNCC345681 |BNCC

Penicillium citrinum

Price： $ 172
number：BNCC345681
Form：
Small filamentous fungi, with obvious colonies on Cha's agar medium, the initial hyphae are white, dense and vigorous, and then produce light gray spores, which spread and grow to the edge of the plate, and the back of the medium is yellow.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Penicillium citrinum

Culture medium	CDA: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water and 6.2±0.2 of pH. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;7-10 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on Cha's agar medium, the initial hyphae are white, dense and vigorous, and then produce light gray spores, which spread and grow to the edge of the plate, and the back of the medium is yellow.
application	Producing citrinin.
Sharing mode	Public welfare sharing

Description

1. Name: Penicillium citrinum

2. BNCC No.: 345681

3. Biosafety level: 4

2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, Czapek ' Sagar: sucrose 30.0g,NaNO3 3.0 g,MgSO4·7H2O 0.5 g,KCl 0.5 g,FeSO4·4H2O 0.01 g,K2HPO4 1.0 g, agar 15.0g, distilled water 1.0 L,pH 6.0-6.5.

2. Atmosphere: aerobic

3. Temperature: 28 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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