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BNCC > Microbial strain > Parabacteroides merdae (Johnson et al. 1986) Sakamoto and Benno 2006 Hahnke et al. 2016
Parabacteroides merdae (Johnson et al. 1986) Sakamoto and Benno 2006 Hahnke et al. 2016-BNCC
  • BNCC
  • Parabacteroides merdae (Johnson et al. 1986) Sakamoto and Benno 2006 Hahnke et al. 2016-BNCC

Parabacteroides merdae (Johnson et al. 1986) Sakamoto and Benno 2006 Hahnke et al. 2016

  • Price： $ 172
  • number：BNCC342254
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
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Parabacteroides merdae (Johnson et al. 1986) Sakamoto and Benno 2006 Hahnke et al. 2016
Culture medium Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37°C;48-72h; Anaerobic
Storage conditions 2-8 ℃
Sharing mode Public welfare sharing
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