BeNa Culture Collection

Trichoderma dark green-BNCC
Trichoderma dark green-BNCC
Trichoderma dark green-BNCC
  • BNCC
  • Trichoderma dark green-BNCC
  • Trichoderma dark green-BNCC
  • Trichoderma dark green-BNCC

Trichoderma dark green

  • Price: $ 115
  • number:BNCC341839
  • Form:
    Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae at the initial stage, dense and vigorous, yellow spores later, full of plates.
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Trichoderma dark green
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 25 ℃-28 ℃;5-7d; Aerobic;
Storage conditions 2-8 ℃
morphology Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae at the initial stage, dense and vigorous, yellow spores later, full of plates.
Separation substrate Dead branches
Sharing mode Public welfare sharing

Description 

1. Name: Trichoderma atroviride P. Karst

2. BNCC No.: 341839

3. Biosafety level:  4

2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions 

1. Medium composition: potato extract 1.0L, glucose 20.0g, agar 15.0g,pH natural. [Note] Potato extract: 200g of peeled potato is taken, cut into small pieces, add 1.0L of water to boil for 30min, filter out potato pieces, and supplement the filtrate to 1.0L.

2. Atmosphere: aerobic

3. Temperature:  25 ℃

4. Notes: 
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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