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Description
1. Name: Trichoderma atroviride P. Karst
2. BNCC No.: 341839
3. Biosafety level: 4
2. Storage conditions: Storage of freezed dried ampoule and agar slant at 2°C to 8°C
3. Growth Conditions
1. Medium composition: potato extract 1.0L, glucose 20.0g, agar 15.0g,pH natural. [Note] Potato extract: 200g of peeled potato is taken, cut into small pieces, add 1.0L of water to boil for 30min, filter out potato pieces, and supplement the filtrate to 1.0L.
2. Atmosphere: aerobic
3. Temperature: 25 ℃
4. Notes: 1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.
2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.
3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.
4.Waste generated from the handling process should be discarded after high-pressure sterilization