BeNa Culture Collection

Massilia neuensis-BNCC
Massilia neuensis-BNCC
  • BNCC
  • Massilia neuensis-BNCC
  • Massilia neuensis-BNCC

Massilia neuensis

  • Price: $ 143
  • number:BNCC341741
  • Form:
    Size: 2-4mm Shape: Round Edge: Neat Transparency: Opaque Color: Light yellow Protuberance: Middle raised surface: Bright and smooth Texture: Wet
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Certificate Related Products
Massilia neuensis
Culture medium R2A agar medium (R2A): tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast extract 0.5g, soluble starch 0.5g, dipotassium bisulfate 0.3g, magnesium sulfate 0.1g, sodium pyruvate 0.3 agar 12 peptone 0.25 glucose 0.5g,pH 7.2±0.2. 121 ℃,15min
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;18-24h; Aerobic;
Storage conditions 2-8 ℃
morphology Size: 2-4mm Shape: Round Edge: Neat Transparency: Opaque Color: Light yellow Protuberance: Middle raised surface: Bright and smooth Texture: Wet
application Taxonomic research, fermentation biological hydrogen production research.
Sharing mode Public welfare sharing

Description 

1. Name: Massilia neuensis  

2. BNCC No.: 341741

3. Biosafety level: 4

2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions 

1, R2A medium: yeast extract (Difco) 0.5g, prion peptone No. 3 (Difco) 0.5g, casein amino acid (Difco) 0.5g, glucose 0.5g, soluble starch 0.5g, sodium pyruvate 0.3g,K2HPO4 ;0.3g,MgSO4·7H2O 0.05g, agar powder 15.0g, disti, adjust pH to 7.2. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature:  30 ℃

4. Notes: 
1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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