Bacillus siam|BNCC341566 |BNCC

Bacillus siam

Price： $ 86
number：BNCC341566
Form：
Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white Protuberance: Middle convex Surface: Rough Texture: Viscous Hard to Pick

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Bacillus siam

Culture medium	Base medium (LB): yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;18-24h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white Protuberance: Middle convex Surface: Rough Texture: Viscous Hard to Pick
application	Production of macrolides
Sharing mode	Public welfare sharing

Bacillus siamensis

Description

1. BNCC No.: 341566

2. Biosafety level: 3

Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1, TSP2: yeast extract 2g, malt extract 2g, glucose 2g, agar 14g(1L).

2. Atmosphere: facultative aerobic

3. Temperature: 28 ℃

Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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