BeNa Culture Collection

Coprinellus radians (Desm.) Vilgalys, Hopple & Jacq. Johnson-BNCC
Coprinellus radians (Desm.) Vilgalys, Hopple & Jacq. Johnson-BNCC
Coprinellus radians (Desm.) Vilgalys, Hopple & Jacq. Johnson-BNCC
  • BNCC
  • Coprinellus radians (Desm.) Vilgalys, Hopple & Jacq. Johnson-BNCC
  • Coprinellus radians (Desm.) Vilgalys, Hopple & Jacq. Johnson-BNCC
  • Coprinellus radians (Desm.) Vilgalys, Hopple & Jacq. Johnson-BNCC

Coprinellus radians (Desm.) Vilgalys, Hopple & Jacq. Johnson

  • Price: $ 143
  • number:BNCC341407
  • Form:
    Small filamentous fungi, with obvious colonies on PDA medium, white in the early stage and light green in the late stage of hyphae, dense and vigorous, spreading to the edge of the plate
Standard strain Quantitative strain DNA extraction
Package:
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Coprinellus radians (Desm.) Vilgalys, Hopple & Jacq. Johnson
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions 25-28 ℃, aerobic, 5-7d
Storage conditions 2-8 ℃
morphology Small filamentous fungi, with obvious colonies on PDA medium, white in the early stage and light green in the late stage of hyphae, dense and vigorous, spreading to the edge of the plate
Separation substrate Collection place: Henan
Sharing mode Public welfare sharing

Coprinellus radians

No. : 341407

Storage conditions : 2~8 ℃

Product format: agar slant in 14mm test tube 

Validity period :growing culture, in 30 dyas

Biosafety level :1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :25-28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.  

Recovery steps:
(1)Prepare 1-2 pieces of PDA plates; 
(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; 
(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; 
(4)Lay flat the small pieces to the center of the agar plate;
(5)Put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

item test results
viability good viability, in 5-7 days strain layer is obvious 
colony morphology small filamentous fungi have obvious colonies, white hyphae, dense and vigorous on the integrated PDA medium, spreading to concentric circles at the edge of the plate and not producing spores,
conclusion good viability, no abnormal colony morphology, qualified
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