Monascus pilosus|BNCC341318 |BNCC

Monascus pilosus

Price： $ 143
number：BNCC341318
Form：
Small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white at the beginning, gradually change to red, purple red after the old, dense and vigorous, spreading to the edge of the plate, and the back of the medium is orange at the beginning, and then becomes purple red.

Standard strain Quantitative strain DNA extraction

Package：

agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Monascus pilosus

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃;3-5 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white at the beginning, gradually change to red, purple red after the old, dense and vigorous, spreading to the edge of the plate, and the back of the medium is orange at the beginning, and then becomes purple red.
application	Scientific research
Sharing mode	Public welfare sharing

Description

1. Name: Monascus pilosus

2. BNCC No.: 341318

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

2. Atmosphere: aerobic

3, Temperature: 28-30 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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