BeNa Culture Collection

Escherichia coli EPEC-BNCC
Escherichia coli EPEC-BNCC
Escherichia coli EPEC-BNCC
  • BNCC
  • Escherichia coli EPEC-BNCC
  • Escherichia coli EPEC-BNCC
  • Escherichia coli EPEC-BNCC

Escherichia coli EPEC

  • Price: $ 115
  • number:BNCC340977
  • Form:
    The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, flat in shape, smooth in surface, bright in surface, moist in texture, easy to stir up, G-(red), bacilli, purity: pure
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Escherichia coli EPEC
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;18-24h; Aerobic;
Storage conditions 2-8 ℃
morphology The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, flat in shape, smooth in surface, bright in surface, moist in texture, easy to stir up, G-(red), bacilli, purity: pure
application Research, Gram-negative bacilli. Containing fasciculus B gene (bfpB) and protein secretion regulatory gene (escV, recommended by GB 4789 National Food Safety Standard Food Microbiological Inspection, GB 4789.6-2016 Diarrhea Escherichia coli Test
Sharing mode Public welfare sharing

Escherichia coli EPEC

Description 

1.BNCC No.:  340977

2. Biosafety level: 3

storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions 

1. nutritious gravy agar: peptone 5.0g, beef extract 3.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH 7.0. [note] adding 5mg MnSO4  ·H2O when culturing Bacillus; it  is beneficial to spore production.

2. Atmosphere:  aerobic

3. Temperature:  37 ℃

Notes: 
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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