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BNCC > Microbial strain > Trichoderma sp.
Trichoderma sp.-BNCC
Trichoderma sp.-BNCC
Trichoderma sp.-BNCC
  • BNCC
  • Trichoderma sp.-BNCC
  • Trichoderma sp.-BNCC
  • Trichoderma sp.-BNCC

Trichoderma sp.

  • Price： $ 115
  • number：BNCC340721
  • Form：
    Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae at the initial stage, dense and vigorous, and yellow spores full of plates later.
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Certificate Related Products
Trichoderma sp.
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;5-7 days; Aerobic;
Storage conditions 2-8 ℃
morphology Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae at the initial stage, dense and vigorous, and yellow spores full of plates later.
application Anti-mold test strain
Sharing mode Public welfare sharing

Description 

1. Name: Trichoderma sp

2. BNCC No.: 340721

3. Biosafety level : 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions 

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

2.. Atmosphere: aerobic

3. Temperature:  28 ℃

Notes: 
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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