Leucosporidium scottii|BNCC340382 |BNCC

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Leucosporidium scottii

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	28 ℃ 5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi have obvious colonies on the comprehensive PDA medium, the initial hyphae are white, sparse and low, and the back of the medium is light yellow.
Sharing mode	Public welfare sharing

Description

1. Name: Leucosporidium scottii

2. BNCC No.:340382

3. Biosafety level: 4

Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. Medium composition: 20% potato liquid 1.0L, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate heptahydrate 1.5g, vitamin B1 8mg, agar 20.0g,pH natural. 20% potato liquid configuration: take 200g of peeled potato, boil for 30min, filter to take filtrate, and make up to 1.0l.

2. Atmosphere: aerobic

3. Temperature: : 28 ℃

Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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