Bacillus mucilaginosus Krassilnikov|BNCC337990 |BNCC

Bacillus mucilaginosus Krassilnikov

Price： $ 86
number：BNCC337990
Form：
Colony diameter is 6-8mm, round, irregular edge, transparent, front white, light color, middle convex, smooth and bright surface, moist texture, easy to pick up, no pigment, G + (blue-purple), bacilli, purity: pure

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

Add to cart

View Cart

Essential Information Certificate Related Products

Bacillus mucilaginosus Krassilnikov

Culture medium	Silicate bacteria medium (SBM): sucrose 5.0g, disodium hydrogen phosphate 2.0g, magnesium sulfate 0.5g, ferric chloride 0.005g, calcium carbonate 0.1g, bauxite 0.5g,pH 7.1±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃; Aerobic; 24-48h
Storage conditions	2-8 ℃
morphology	Colony diameter is 6-8mm, round, irregular edge, transparent, front white, light color, middle convex, smooth and bright surface, moist texture, easy to pick up, no pigment, G + (blue-purple), bacilli, purity: pure
application	Bacterial fertilizer
Sharing mode	Public welfare sharing

BacillusmucilaginosusKrassilnikov

1. BNCC No.: 337990

2. biosafety level: 4

Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions

1. silicate bacteria medium: sucrose 5.0g Na₂HPO₄2.0g MgSO₄·H₂O 0.5g FeCl₃ 0.005g CaCO₃ 0.1g soil mineral 1.0g Agar 15.0g Distilled water 1.0 L pH 7.0-7.2,121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature:30 ℃

Notes:

1. Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2. Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3. Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4. Waste generated from the handling process should be discarded after high-pressure sterilization.

Download certificate