Streptomyces avidin|BNCC337586 |BNCC

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Streptomyces avidinii

Culture medium	Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	30 ℃;2-3 days; Aerobic
Storage conditions	2-8 ℃
morphology	G + bacillus size: 2-4mm shape: round edge: irregular transparency: opaque color: white bulge: middle bulge surface: bright texture: dry
Separation substrate	Soil
application	Type strain; Produce antibiotic MSD-235
Sharing mode	Public welfare sharing

1.Description

1. Name:Streptomyces avidinii

2. BNCC No.:337586

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth conditions

1. ISP-2 medium: yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, agar 15.0g, distilled water 1.0 L,pH7.3. 121 ℃,15 minutes sterilization.

2. Atmosphere: aerobic

3. Temperature: 28 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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