BeNa Culture Collection

Fusarium nivale-BNCC
Fusarium nivale-BNCC
Fusarium nivale-BNCC
  • BNCC
  • Fusarium nivale-BNCC
  • Fusarium nivale-BNCC
  • Fusarium nivale-BNCC

Fusarium nivale

  • Price: $ 115
  • number:BNCC337556
  • Form:
    Small filamentous fungi, with obvious colonies on the integrated PDA medium, white hyphae, dense and low flat, spreading to the edge of the plate, no spores, and light yellow on the back of the medium.
Standard strain Quantitative strain DNA extraction
Package:
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Fusarium nivale
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0 0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions 28 ℃;5-7 days; Aerobic
Storage conditions 2-8 ℃
morphology Small filamentous fungi, with obvious colonies on the integrated PDA medium, white hyphae, dense and low flat, spreading to the edge of the plate, no spores, and light yellow on the back of the medium.
Separation substrate Snow rot wheat
Sharing mode Public welfare sharing

Fusarium nivale.

Storage conditions: 2~8 ℃

No.: 337556

Product format: agar slant in 14mm test tube 

Validity period: Activated form within 30 days

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions:28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

 

item test result
viability good viability, in 5-7 days strain layer is obvious
colony morphology small filamentous fungi have obvious colonies, white hyphae, dense and low flat on the integrated PDA medium, spreading to the edge of the plate without spores, and the back of the medium is light yellow.
conclusion good viability, no abnormal colony morphology, qualified
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