BeNa Culture Collection

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BNCC > Microbial strain > Bacillus subtilis
Bacillus subtilis-BNCC
Bacillus subtilis-BNCC
  • BNCC
  • Bacillus subtilis-BNCC
  • Bacillus subtilis-BNCC

Bacillus subtilis

  • Price： $ 100
  • number：BNCC337420
  • Form：
    Size: 2-4mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Grey White Protuberance: Flat Surface: Rough Grey Dark Texture: Hard Dry
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Certificate Related Products
Bacillus subtilis
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;18-24h; Aerobic
Storage conditions 2-8 ℃
morphology Size: 2-4mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Grey White Protuberance: Flat Surface: Rough Grey Dark Texture: Hard Dry
Sharing mode Public welfare sharing

1.Description

1.Name: Acetobacter pasteurianus

2.BNCC No.: 337420

3.Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Glucose agar: yeast extract 5.0g bacteria adjust pH to 6.8 with peptone 3.0g glucose 30.0g calcium carbonate 10.0g agar 15.0g distilled water 1.0L.

2.Atmosphere: aerobic

3.Temperature: 25 ℃

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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