Microlunatus phosphovorus|BNCC337414 |BNCC

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Microlunatus phosphovorus

Culture medium	Small moon bacteria medium (DSM MEDIUM 776.)(MICROLUNATUS MEDIUM): glucose 0.5g, peptone 0.5g, yeast powder 0.5g,Na-glutamate0.5g,KH2PO4 0.5g, (NH4)2SO4 0.1g,MgSO4 x 7 H2O 0.1g, agar 20.0g (without liquid medium), distilled water 1000.0ml,PH7.0
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;3-5 days; Aerobic
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white Protuberance: Middle convex Surface: Smooth Texture: Wet
Separation substrate	Activated sludge, Japan
Sharing mode	Public welfare sharing

Microlunatus phosphovorus

Storage conditions : 2~8 ℃

No. : 337414

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30 ℃, aerobic, 776. MICROLUNATUS MEDIUM,3-5 days. 776. MICROLUNATUS MEDIUM: glucose 0.5g, peptone 0.5g, yeast powder 0.5g,Na-glutamate0.5g,KH2PO4 0.5g, (NH4)2SO4 0.1g,MgSO4 x 7 H2O 0.1g, agar 20.0g (not included in liquid medium), distilled water 1000.0ml,PH7.0

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 3-5 days liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: middle raised surface: smooth texture: moist
Conclusion:	good viability, no abnormal colony morphology, qualified

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