Malassezia furfur|BNCC337308 |BNCC

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Malassezia furfur

Culture medium	Pityrosporum culture medium (MFM): wort agar 60.0g, bovine bile (dry) 20.0g, Tween 40 10.0g,Glycerol mono-oleate 2.5g, distilled water 1.0L. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;24-48h; Aerobic
Growth characteristics	Note: The original unit of this bacterium is a secondary bacterium. Attention to Follow-up Instructions
Storage conditions	2-8 ℃
morphology	Round, neat edges, opaque, milky white on the front, golden yellow on the back, convex in the middle, smooth and bright surface, moist texture, easy to stir up, no pigment, G + (blue-purple), cocci, purity: pure
application	Biomedical research
Sharing mode	Public welfare sharing

1.Description

1.Name: Malassezia furfur(Pityrosporum orbiculare)

2.BNCC No.: 337308

3.Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Pityrosporum culture medium: malt juice agar 60.0g bovine bile (dry) 20.0g Tween 40 10.0g Glycerol mono-oleate 2.5g distilled water 1.0 L. 121 ℃,15min.

2.Atmosphere: aerobic

3.Temperature: 28-30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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