Rhodobacter sphaeroides|BNCC337046 |BNCC

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Rhodobacter sphaeroides

Culture medium	Fan's medium (112VY):K2HPO4 1.0g,MgSO4 0.5g, yeast extract 10.0g, agar 20.0g, distilled water 1000 ml,pH 7.0-7.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;48-72h; Aerobic
Growth characteristics	Orange-yellow colonies.
Storage conditions	2-8 ℃
morphology	The colony diameter is 0.5-1mm, round, with neat edges, opaque, orange-yellow front, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G-(red), cocci
Separation substrate	Pond water, India
application	Reduce molasses wastewater
Sharing mode	Public welfare sharing

Rhodobacter sphaeroides

Storage conditions : 2~8 ℃

No. : 337046

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30 ℃, anaerobic, 112 medium, 48-72h. 112 medium: k ₂HPO₄1.0g, MgSO₄ 0.5g, yeast extract 10.0g, agar 20.0g (not included in liquid medium), distilled water 1000 ml, pH 7.0-7.2.

Recovery steps:

(1)Prepare a φ18mm test tube of 10ml liquid medium(put in an anaerobic environment for deoxygenation in advance);

(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Put the test tube under the above culture conditions for cultivation. The culture solution is obviously turbid or mass growth at the solution bottom occur, this indicates the bacterial grows well.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 48-72h the liquid medium become turbid, and the strain grwo slowly on the plate
Colony morphology: (pictured above)	ball red bacteria, gram-negative spores, 112 liquid culture bacterial fluid turbid, plate strain growth is weak, Liquid culture is recommended.
Conclusion:	good viability, no abnormal colony morphology, qualified

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