Burkholderia cepacia|BNCC337012 |BNCC

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Burkholderia cepacia

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a liquid suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	30 ℃;24-48h; Aerobic
Storage conditions	2-8 ℃
morphology	Size: 0.5-1mm shape: round edge: neat transparency: opaque Color: yellow uplift: middle convex surface: bright and smooth texture: moist and viscous
application	Type strain, determination of antibacterial preservative content, medium test, onion-producing burkocin, quality control strain
Sharing mode	Public welfare sharing

Burkholderia cepacia

Storage conditions : 2~8 ℃

No. : 337012

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30 ℃, aerobic, nutritious agar, 18-24h. Nutritional agar: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 15.0g (not included in liquid medium), distilled water 1.0L,pH7.0,121 ℃,15min sterilization.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 18-24h liquid medium become turbid, strain layer is obvious; colony is typical on marked plate
Colony morphology: (pictured above)	Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: yellow-green uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability, no abnormal colony morphology, qualified

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