Metarhizium pingshaense Chenet Guo(1986)|BNCC336563 |BNCC

Metarhizium pingshaense Chenet Guo(1986)

Price： $ 115
number：BNCC336563
Form：
Small filamentous fungi have obvious colonies on the comprehensive PDA medium, the initial hyphae are white, dense and vigorous, and then produce yellowish green spores, which spread and grow to the edge of the plate, and the back of the medium is yellow.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Metarhizium pingshaense Chenet Guo(1986)

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;7-10 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi have obvious colonies on the comprehensive PDA medium, the initial hyphae are white, dense and vigorous, and then produce yellowish green spores, which spread and grow to the edge of the plate, and the back of the medium is yellow.
application	Control of larvae and adults of Coleoptera and Homoptera.
Sharing mode	Public welfare sharing

Metarhizium pingshaense Chen et Guo

Description

1. BNCC No.:336563

2. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. integrated PDA potato juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g, the pH is natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

2. Atmosphere:aerobic

3. Temperature: 24-28 ℃

Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for long time.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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