Enterococcus faecalis (Andrewes et Horder) Schleifer et kilpper-Balz|BNCC336479 |BNCC

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Enterococcus faecalis (Andrewes et Horder) Schleifer et kilpper-Balz

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;18-24h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 0.5-1mm Shape: round Edge: neat Transparency: Opacity Color: off-white Protuberance: Middle bulge Surface: bright and smooth Texture: moist and easy to stir up
application	Lactic acid bacteria preparation
Sharing mode	Public welfare sharing

Enterococcus faecalis

Storage conditions : 2~8 ℃

No. : 336479

Product Format : freeze dried, 200ul

Validity period :6 years

Biosafety level : 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture :30°C, aerobic, nutrient gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of NB liquid media or two NA agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min).

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability. in 20 hours NB liquid medium become turbid, obvious strain layer occurs on the plate; colony is typical on marked plate
colony morphology	size: general color: milky white shape: round edge: neat edge dry and wet: moist and smooth: smooth transparency: opaque uplift: uplift
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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