Rhodopseudomonas palustris|BNCC336448 |BNCC

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Rhodopseudomonas palustris

Culture medium	ATYP agar (ATYP): ammonium chloride 1.0g; 0.5g of potassium dihydrogen phosphate; Magnesium chloride 0.5g; 0.1g of calcium chloride; 1.0g of sodium chloride; 2.0g of sodium acetate; 1.0g of sodium succinate; Yeast paste powder 0.5g; Peptone 0.5g; Ferrous chloride 1.8mg; Cobalt chloride 0.25mg; Nickel chloride 0.01mg; Copper chloride 0.01mg; Manganese chloride 0.07mg; Zinc chloride 0.1mg; biotin 0.01mg; Niacin 0.035mg; Calcium pantothenate 0.01mg; Thiamine hydrochloride 0.03mg;VB12 0.005mg; Pyrazidomide hydrochloride 0.01mg; p-aminobenzoic acid 0.02mg; Agar 13.0g (not included in liquid medium); Distilled water 1L. pH 6.8. 115 ℃,30min. (Because sodium selenite is easy to decompose when heated, the product becomes slightly red and turbid after high-temperature sterilization). After sterilization, please shake well to prevent agar from being deposited on the bottom of the vessel and solidify, pour it into a plate and set for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃,3-4 days, anaerobic light
Growth characteristics	Photosynthetic bacteria
Storage conditions	2-8 ℃
morphology	Round, neat edges, opaque, front white, dark, convex in the middle, smooth and bright surface, moist texture, easy to stir up, no pigment, G-(red), bacilli, purity: pure
application	Research; Production. It is used in the production of lycopene, water purification and as a feed additive.
Sharing mode	Public welfare sharing

1、Description

1.Name: Rhodopomonas palustris

2. BNCC No：336448

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, 112 Van Niel ' S Yeast Agar:K2HPO4 1.0 g,MgSO4 0.5g, yeast extract 10.0 g, distilled water 1000 ml,pH7.0-7.2. 121 ℃,15min.

2.Atmosphere: anaerobic, cultured under tungsten lamp

3. Temperature: 30 ℃

4.Culture method: fill 15-20mL screw test tube with liquid culture medium and sterilize for later use. Inoculation: In the safety cabinet, after absorbing 0.3-0.5mL of culture medium to dissolve the bacterial powder, all of them are beaten back into the liquid culture medium and sealed tightly. Let it stand in the incubator, illuminate the incubator for 5 days, observe the turbidity of the liquid, and the color is dark red, that is, the activation is successful.

4、Notes:

1.Normal culturing time, 1-2 days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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