Acetobacter sp.|BNCC336433 |BNCC

Essential Information Certificate Related Products

Acetobacter sp.

Culture medium	Acetic acid bacteria medium (AM): glucose 100g, yeast extract 10g,CaCO3 20g, agar 15g, distilled water 1.0L. pH 6.8. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;3-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	The colony diameter is 1-2mm, round, with irregular edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, smooth and bright surface, moist texture, easy to stir up, G-(red), bacilli, purity: pure
application	Ethanol Oxidation to Produce Acetic Acid
Sharing mode	Public welfare sharing

Acetobacter sp.

Storage conditions : 2~8 ℃

No. : 336433

Product Format : freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth Conditions:30 ℃, aerobic, acetic acid bacteria medium, 3-7d. Acetic acid bacteria medium: glucose 100g, yeast extract 10g,CaCO3 20g, agar 15g (liquid medium does not contain), distilled water 1.0L. pH 6.8. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability, in 3-7days liuquid medium become turbid, strain layer is obvious ; colony is typical on the streaked plate
colony morphology: (above)	size: 1-2mm shape: round edge: neat transparency: opaque color: off-white uplift: middle raised surface: bright and smooth texture: moist and viscous
conclusion	good viability, no abnormal colony morphology, qualified

Download certificate